Notes from NEET topper
Criteria of Good Vectors:
-
Size: A good vector should be of an appropriate size, neither too large nor too small, to accommodate the inserted DNA without affecting its stability.
-
Origin of Replication: Vectors must have a well-defined origin of replication, ensuring that the inserted DNA can be replicated in the host cell.
-
Selectable Markers: Vectors should contain selectable markers, such as antibiotic resistance genes, to help identify and select cells that have successfully taken up the vector.
-
Multiple Cloning Sites (MCS): A vector should have an MCS with unique restriction enzyme recognition sites, making it easy to insert DNA fragments at specific locations.
-
Stability: Good vectors should be stable, ensuring that the inserted DNA is retained during cell division and replication.
-
Expression Elements: In some cases, vectors may need to contain promoter and regulatory elements for the expression of inserted genes.
